Immunological properties of Escherichia coli heat-stable enterotoxins: development of a radioimmunoassay specific for heat-stable enterotoxins with suckling mouse activity
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چکیده
منابع مشابه
Construction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated Form of the Heat-Stable Enterotoxins in Escherichia coli
Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
متن کاملEscherichia coli contains plasmids coding for heat-stable b, other enterotoxins, and antibiotic resistance.
Plasmid DNAs obtained from 18 Escherichia coli isolates that hybridized with the heat-stable b (ST-b) enterotoxin gene probe were examined by Southern blot analysis for genes coding for heat-labile, ST-a, and ST-b enterotoxins with specific radiolabeled DNA probes. Four E. coli isolates contained plasmids coding for both heat-labile and ST-b enterotoxins, and one isolate contained a plasmid cod...
متن کاملDoes enteropathogenic Escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b, or cholera toxin A subunits?
Although most enteropathogenic Escherichia coli strains do not produce recognized enterotoxins, we wished to examine whether they produce any factors like heat-stable enterotoxin b or cholera toxin active subunits that might be missed by conventional assay methods. E. coli strains E851 (O142) and E2348 (O127) that had caused diarrhea in volunteers were negative for heat-labile enterotoxin and h...
متن کاملDifferences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins of Escherichia coli.
The response of adult rabbit small intestine to the heat-stable (ST) and heat-labile (LT) enterotoxins of Escherichia coli has been investigated by employing the ligated loop technique. Fluid accumulation was determined in relation to enterotoxin dose and duration of gut exposure. The individual responses to ST and LT differed in a characteristic manner. Onset of net fluid accumulation in respo...
متن کاملconstruction and expression of a fused gene for b subunit of the heat-labile and a truncated form of the heat-stable enterotoxins in escherichia coli
elaboration of different toxins by enterotoxigenic e. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. various strategies have been employed to raise antibodies against these toxins as a line of defense. in this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1981
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.33.1.193-198.1981